1 Txoj kev smear yog ib txoj hauv kev ua ib zaj duab xis uas sib npaug ntawm cov ntaub ntawv ntawm aiav swb. Smear cov ntaub ntawv muaj xws li cov kab mob ib leeg, me me algae, ntshav, kab mob kab mob kua, xoob cov ntaub so ntswg ntawm cov tsiaj thiab nroj tsuag, testis, anthers, thiab lwm yam.
Ua tib zoo saib thaum smearing:
(1) Lub iav swb yuav tsum yoghuv si.
(2) Lub iav swb yuav tsum tiaj tus.
(3) Cov txheej txheej yuav tsum tsis sib xws. Cov kua smear yog poob rau sab xis ntawm nruab nrab ntawm cov swb, thiab kis tusyees nrog ib rab riam los yog txhuam hniav.
(4) Cov txheej yuav tsum nyias nyias. Siv lwm tus swb ua tus thawb, thiab maj mam thawb los ntawm sab xis mus rau sab laug raws li qhov chaw ntawm cov swb uas cov tshuaj smear tau poob (lub kaum sab xis ntawm ob qhov swb yuav tsum yog 30 ° -45 °), thiab siv cov txheej nyias nyias.
(5) Tsau. Rau fixation, tshuaj fixative los yog qhuav txoj kev (cov kab mob) yuav siv tau rau fixation.
(6) Dyeing. Methylene xiav yog siv rau cov kab mob, Wright's stain yog siv rau cov ntshav, thiab qee zaum iodine siv tau. Cov tshuaj dyeing yuav tsum npog tag nrho cov pleev xim rau saum npoo.
(7) Yaug. Soak qhuav nrog daim ntawv absorbent los yog toast qhuav.
(8) Kaw zaj duab xis. Txhawm rau khaws cia ntev, kaw cov swb nrog Canadian cov pos hniav.
2. Cov txheej txheem ntsiav tshuaj yog ib txoj hauv kev ua cov ntawv los ntawm kev muab cov ntaub ntawv lom neeg nruab nrab ntawm cov iav swb thiab lub hau npog thiab siv qee qhov siab kom tawg mus rau cov ntaub so ntswg.
3. Txoj kev Mounting yog ib txoj hauv kev uas cov ntaub ntawv lom neeg raug kaw tag nrho los ua cov qauv swb. Txoj kev no tuaj yeem siv los ua ib ntus lossis ruaj khov. Cov khoom siv thauj khoom muaj xws li: cov kab mob me me xws li Chlamydomonas, Spirogyra, Amoeba, thiab nematodes; Hydra, nplooj epidermis ntawm cov nroj tsuag; tis, taw, qhov ncauj ntawm kab, tib neeg lub qhov ncauj epithelial hlwb, thiab lwm yam.
Kev saib xyuas yuav tsum tau them rau qhov kev npaj ntawm txoj kev swb:
(1) Thaum tuav lub swb, nws yuav tsum tiaj tus lossis muab tso rau ntawm lub platform. Thaum cov dej ntws tawm, cov dej yuav tsum tsim nyog, kom nws tsuas yog npog los ntawm lub khob iav.
(2) Cov khoom yuav tsum tau nthuav tawm nrog ib rab koob los yog tweezers tsis sib tshooj, thiab flattened rau tib lub dav hlau.
(3) Thaum muab cov iav npog, maj mam npog cov dej ntws los ntawm ib sab kom tsis txhob muaj cov npuas npuas tshwm.
(4) Thaum staining, muab ib tug staining tov rau ntawm ib sab ntawm lubnpog iav, thiab nqus nws los ntawm sab nraud nrog cov ntawv nqus dej kom cov qauv hauv qab lub khob iav sib npaug. Tom qab cov xim, siv tib txoj kev, tee ib tee dej, nqus cov tshuaj staining, thiab saib xyuas hauv lub tshuab tsom.
Post lub sij hawm: Nov-22-2022