Pipettes feem ntau siv nyob rau hauv lub chaw soj nstuam kom hloov cov milliliter ntim ntawm cov kua, los ntawm qhov tsawg kawg nkaus ntawm 1 ml mus rau qhov siab tshaj plaws ntawm 50 ml. Straws tuaj yeem muab pov tseg hauv cov yas tsis muaj menyuam lossis rov siv dua hauv autoclavable iav. Ob lub pipettes siv lub pipette los nqus thiab tshem tawm cov kua. Qhov ntau thiab tsawg ntawm pipettes yog siv nyob rau hauv kev sim sib txawv nrog tib pipette. Piv txwv li, pipettes yog ib qho tseem ceeb rau kev sib tov tshuaj tov los yog cell suspensions, hloov cov kua ntawm txawv ntim, los yog plating reagents ntawm ntau ntom. Ntev npaum li kev ceev faj yog them rau qhov ntim ntawm cov kua aspirated thiab tshem tawm, pipettes tuaj yeem yog cov cuab yeej tseem ceeb hauv chav kuaj kom raug hloov cov milliliter ntim cov kua.
Hom pipettes thiab cov khoom tseem ceeb ntawm pipettes
Pipettes feem ntau yog sterile ib leeg-siv yas raj; lawv tuaj yeem yog autoclavable, reusable iav raj.
Txhua pipettes siv lub pipette thaum pipettes.
Lub pipette tshem tawm qhov xav tau rau cov kws tshawb fawb kom pipette los ntawm qhov ncauj zoo li ua ntej. Txoj kev siv lub hauv paus chiv keeb tsis pom zoo vim tias nws muaj peev xwm ua rau muaj qhov tshwm sim loj rau cov kua dej nqus rau hauv lub qhov ncauj.
Lub pob pipette yog ib hom pipette nrog qhov tsis zoo tshaj plaws. Nws feem ntau yog ua ke nrog lub iav pipette kom hloov cov kua dej sib txawv.
Pipette twj tso kua mis kuj tseem haum rau iav pipettes, uas tuaj yeem hloov cov kua ntim ntau dua. Pipette twj tso kua mis feem ntau haum rau dispensing tib lub ntim ntawm cov kua ntau zaus.
Assistant pipettes yog cov kav dej ntau tshaj plaws. Nws muaj ob peb qhov tseem ceeb: lub qhov ncauj qhov ncauj yog qhov chaw tso cov pipette thiab qhov chaw lim dej tso rau hauv, uas tiv thaiv sab hauv ntawm tus pab pipette los ntawm kev sib kis ntawm cov kua.
Ob lub nyees khawm tuaj yeem pom ntawm tus kov ntawm tus pab pipette. Thaum nias lub khawm sab sauv, cov kua yog aspirated, thiab thaum lub khawm qis yog nias, cov kua tawm tawm.
Feem ntau tus pab pipettes kuj muaj lub qhov rooj tswj cov kua dej tawm. Piv txwv li, nws tuaj yeem raug teeb tsa kom tso tawm cov kua hauv lub siab, lossis nws tuaj yeem teem rau lub ntiajteb txawj nqus tsis muaj zog sab nraud.
Txawm hais tias qee tus pab pipettes tuaj nrog lub hwj chim qaum, feem ntau yog siv roj teeb.
Qee tus neeg pabcuam pipettes tuaj nrog lub sawv ntsug uas haum rau hauv qhov chaw kov, uas tso cai rau tus neeg pabcuam pipette muab tso rau ntawm nws sab thaum tsis siv yam tsis tau tshem lub pipette.
Raws li tau hais ua ntej, tib lub pipette tuaj yeem siv ntau qhov sib txawv ntawm cov pipettes nyob ntawm qhov ntim yuav tsum tau pipetted, los ntawm tsawg li 0.1 milliliters mus rau ntau li kaum milliliters.
Basic kev ua haujlwm ntawm pipettes
Ua ntej, xaiv qhov loj pipette raws li qhov ntim ntawm cov kua koj xav hloov. Tom qab ntawd qhib lub pob los ntawm sab saum toj, kov tsuas yog ib feem saum toj no lub cim zuam, ntxig rau hauv lub ntsis ntawm lub pipette, thiab tshem tawm cov pob ntxiv.
Tom ntej no, tuav lub pipette nrog ib txhais tes thiab qhib lub hau ntawm lub thawv uas muaj cov kua koj xav kom aspirate. Ua kom lub pipette upright, maj mam nias lub khawm sab sauv kom maj mam nqus koj cov qauv.
Siv cov kab kawm tiav ntawm phab ntsa pipette los ntsuas qhov ntim ntawm cov kua koj xav hloov. Nco ntsoov tias lub ntim yuav tsum tau nyeem nyob rau hauv qab ntawm lub meniscus, tsis yog saum toj.
Tom qab ntawd ua tib zoo tso cov kua rau hauv lub thawv uas koj xaiv, saib xyuas kom tsis txhob cia lub pipette taub hau kov txhua qhov chaw tsis muaj menyuam.
Siv ceev faj thiab maj mam quab yuam thaum tshem tawm cov kua, tshwj xeeb tshaj yog thaum siv cov ntim ntim me me, kom tsis txhob kis tus neeg pab cuam pipette lim thiab cov qauv, lossis ua rau cov neeg pab cuam lub pipette puas. Kev ua yuam kev thaum siv tus pab pipette tuaj yeem ua rau lwm tus neeg muaj kev paub dhau los hauv chav kuaj, uas tej zaum yuav tau muab cov pipette sib nrug los kho. Thaum siv cov kua dej ntau lossis cov kua tawm tawm, cov kua hloov ceev tuaj yeem nce ntxiv los ntawm nias lub pob nyuaj.
Thaum kawg, nco ntsoov muab pov tseg kom zoo tom qab hloov cov kua.
Daim ntawv thov
Tam sim no koj paub yuav ua li cas khiav lub pipette, cia peb saib kom meej ntxiv ntawm qee cov ntawv thov kev sim.
Ib kauj ruam tseem ceeb thaum culturing thiab plating hlwb yog uniform faib ntawm cov hlwb nyob rau hauv lub kawg daws. Kev tshem tawm ntawm tes tuaj yeem sib tov maj mam thiab siv tau zoo siv lub pipette, uas ib txhij sib tov tshuaj tov thiab reagents.
Tom qab kev rho tawm lossis ua cov kev sim ntawm cov hlwb, cov pipettes tuaj yeem siv los hloov tag nrho cov cell clones rau kev nthuav dav lossis kev soj ntsuam tom ntej.
Post lub sij hawm: Aug-31-2022